Programme 2010

Conference Program HTPD10, Krakow, Poland

 Monday Oct 4, 2010
Welcome reception
Introductory remarks
Plenary lecture: High-Throughput Techniques for Small Molecule Drug Discovery
and Early Development: Lessons Learned

Speaker: Christof Fattinger, F. Hoffmann-La Roche Ltd, Basel, Switzerland
Tuesday Oct 5, 2010

Session 1. Case studies 1

101 HTS Supports Process Characterization in Downstream Process Development
Susanne Nath, Katharina Doninger, Annika Kleinjans and Frank Zettl*
Pharma Biotech Production & Development, Roche Diagnostics GmbH, Penzberg, Germany
102 Studying Host Cell Protein Interactions using High Throughput Protein A Affinity Chromatography
Vikram N. Sisodiya*, Paul J. McDonald, Kathlyn Lazzareschi, Maricel Rodriguez and Robert Fahrner
Early Stage Purification, Genentech, Inc., South San Francisco, CA, U.S.A.
103 Rapid Downstream Process Development using TECAN HTS System
Edo Prašnikar
Lek Pharmaceuticals, Sandoz Co., BPO Novartis, Mengeš, Slovenia
104 High-Throughput Screening of Excipients Effective Against Antigen Aggregation at Air-Water Interface
Sébastien Dasnoy*1, Nancy Dezutter2, Dominique Lemoine2, Vivien Le Bras2 and Véronique Préat1
1 Université catholique de Louvain, Louvain Drug Research Institute, Brussels, Belgium
2 GlaxoSmithKline Biologicals s.a., Research & Development, Rixensart, Belgium
105 Process Characterization to Establish a Control Strategy for Management of Chromatography Resin Lot-To-Lot Variability
Tryggve Bergander, Mattias Ahnfelt, Gunnar Malmquist, Eggert Brekkan*, Catharina Hemström Nilsson and Karol Lacki
GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden
106 Using Robotics and High Throughput Screening to Develop Early Stage Purification Processes 
Paul McDonald*(1), Nick Cai(1), Joseph Cheng(1), Ivy Lin(1), Marc Wong(1), Jennifer Hopp(2), Philip Lester(1) and Brian Kelley(1)
1 Bioprocess Development and
2 Oceanside Process Development, Genentech, Inc., South San Francisco, CA, U.S.A.


Lunch break

Session 2. Design of experiments and data mining

201 Application of Genetic Algorithms in High Throughput Process Development
Katrin Treier*1, Anna Osberghaus1, Philip Lester2, Eric von Lieres3 and Jürgen Hubbuch1
1 Institute of Engineering in Life Sciences, University of Karlsruhe, Germany
2 Early Stage Process Development, Genentech South San Francisco, U.S.A.
3 Institute of Biotechnology 2, Research Center Jülich, Germany
202 High-Throughput Isotherm Determination and Thermodynamic Modeling of Protein Adsorption on Mixed-Mode Chromatographic Resins
B.K. Nfor1, M. Noverraz2, S.Chilamkurthi *1, P.D.E.M. Verhaert1, L.A.M. van der Wielen1 and M. Ottens1
1 Delft University of Technology, Department of Biotechnology, Delft, The Netherlands
2 École Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Laboratory of Cellular Biotechnology, Lausanne, Switzerland
203 Innovative Automated Small Scale Parallelized Biochromatography
Dietmar A. Lang
Lonza Biologics plc, Slough, Berkshire, U.K.
204 An Analytical Approach to Batch Uptake Characterization of Chromatography Media
Peter Sandblad*, Anneli Florén, Ann-Katrin Hellman and Tryggve Bergander
GE Healthcare Bio-Sciences AB, Uppsala, Sweden


Coffee break

Session 3. Eliminating analytical challenges

301 Specific Protein Quantification for Inline and Offline Applications using Multivariate Calibration Methods
Sigrid Hansen* and Jürgen Hubbuch
Karlsruhe Institute of Technology, Institute of Process Engineering in Life Sciences, Biomolecular Separation Engineering, 76131 Karlsruhe, Germany
302 Holistic Approaches to Improving the Throughput of Analytical Methods to Support Therapeutic Monoclonal Antibody Process Development
Dell Farnan*, Tony Moreno, Jennifer Rea, and John T. Stults
Genentech Inc., Protein Analytical Chemistry, South San Francisco, CA, U.S.A.
303 High Throughput Analytical Methods for Enabling Downstream Bioprocess Development
Judy Chou*, Daniela Olszova, Maria Darlucio, Ross Pritchett, Jian Zhang and Diya Ren
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, U.S.A.
304 Strategic Assay Deployment in High Throughput Process Development
Spyridon Konstantinidis*1, Eva Heldin2, Sunil Chhatre1 and Nigel Titchener-Hooker1
1The Advanced Centre for Biochemical Engineering, Dept of Biochemical Engineering, University College London, London, U.K.
2 GE Healthcare Bio-Sciences AB, Uppsala, Sweden


Poster session with refreshments
 Wednesday Oct 6, 2010
Panel discussion; Automation challenges

Session 4. Scale-up and scale-down studies

Calibration of Mathematical Models of Preparative Chromatography
Niklas Borg1, Line Noami Lund2, Bernt Nilsson1* and Arne Staby2
1 Lund University, Dept. of Chemical Engineering, Lund, Sweden
2 Novo Nordisk A/S, Gentofte, Denmark
The Challenges of Scale-Up and High-Throughput Methods in Downstream Development
Michael Dieterle*, Joey M. Studts, Thomas Rathjen, Denise Wenzel and Dorothee Ambrosius
Boehringer Ingelheim Pharma GmbH & Co. KG, Biopharm Biopharmaceuticals Cell Culture & DP, Downstream Development, Germany
The Use of Microwell-Based Mimics for the Rapid Evaluation of Interactions Between Primary Recovery Unit Operations for a Typical Mammalian Cell Bioprocess
Andrew Tait*, Jean Aucamp, Simyee Kong, Dan Bracewell and Mike Hoare
University College London, Department of Biochemical Engineering, London, U.K.
Achieving Comparability Between Batch Binding and Packed Column Methods
Debbie O’Connor, Scientist
Genentech, Inc, Late Stage Purification, Process R&D, CA, U.S.A.
Combination of HTE and Mechanistic Modeling for a Rapid and Highly Predictive Optimization of an Ion Exchange Chromatography Step
Anna Osberghaus*, Susanne Nath, Eric von Lieres and Jürgen Hubbuch
Karlsruhe Institute of Technology, Institute of Process Engineering in Life Sciences, Biomolecular Separation Engineering, Karlsruhe, Germany


Lunch and trip

Session 5. Beyond early screening: The future of HTPD

Past, present and (potential) future of HTPD
Jürgen Hubbuch
Institute of Engineering in Life Sciences, University of Karlsruhe, Germany
More Meaningful Data Instead of Just More Data: using Advanced Experimental Designs Combined with High Throughput Methods
Wolfgang. Berthold
Biogen Idec, Zug, Switzerland
Downstream Process Development in Five Days
Jonathan Coffman*, Jeffrey Salm, Aaron Noyes and Ranga Godavarti
Pfizer, U.S.A.
The Next Decade of HTPD: Evolution, or Revolution?
Brian Kelley, Vice President
Genentech Inc., South San Francisco, CA, U.S.A.


Closing Dinner
 Thursday Oct 7, 2010

Session 6: Case studies 2

601 Next Generation BioFuels: Analytical Tools, Automation Challenges and Screening Technologies
Masood Hadi
Director Technologies Division,Joint BioEnergy Institute, Emeryville, CA and Senior Member of Technical Staff, Biomass Sciences and Conversion Technologies Department, Sandia National Laboratories, Livermore, CA, U.S.A.
602 High Throughput Screening for the Identification of Unique Selectivity Windows in Hydroxyapatite and Multimodal Chromatographic Systems
C. J. Morrison, M. Holstein and S.M. Cramer*
Rensselaer Polytechnic Institute, Troy, NY, U.S.A.
603 Development of a Capture Chromatographic Step for Purification of Pro-Insulin Expressed in E.Coli
E.Heldin*1, J. Shanagar1, S. Grönlund1, E. Hallgren1, K. Eriksson1, L. Vilela2, H. Tunes2 and M. Xavier2
1GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden
2BIOMM S.A. Belo Horizonte, Brazil
604 Review and Outlook on Automated Small Scale Parallelized Biochromatography
Jürgen Friedle
Atoll GmbH, Ettishofer Straße 10, D-88250 Weingarten, Germany
605 The Use of High Throughput Process Development for the Process Optimisation for Fab Purification
Chloe Booth, Bastiaan Lobbezoo and Sharon Williams*
ProMetic BioSciences Ltd, 211 Cambridge Science Park, Milton Road, Cambridge, CB4 0WA, U.K.
606 High Throughput Downstream Screening System for Protein Purification Using Membrane Adsorbers
Martin Leuthold* and Rene Faber
Sartorius Stedim Biotech GmbH, Göttingen, Germany


Closing remarks